21,175 Views
•
13:16 min
•
January 22, 2018
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific for the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) genes. The method is suited for reliable and sensitive detection of DNA contamination in most eukaryotes and prokaryotes.
Powiązane Filmy
08:40
Methyl-binding DNA capture Sequencing for Patient Tissues
Powiązane Filmy
8520 Views
13:21
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Powiązane Filmy
9820 Views
12:24
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
Powiązane Filmy
9248 Views
10:36
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Powiązane Filmy
11916 Views
12:11
Methodology for Accurate Detection of Mitochondrial DNA Methylation
Powiązane Filmy
13207 Views
08:36
ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
Powiązane Filmy
12504 Views
11:22
High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Powiązane Filmy
17847 Views
11:27
Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
Powiązane Filmy
9326 Views
07:30
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
Powiązane Filmy
11864 Views
08:35
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Powiązane Filmy
19502 Views
Read Article
Cytuj ten artykuł
Hashemipetroudi, S. H., Nematzadeh, G., Ahmadian, G., Yamchi, A., Kuhlmann, M. Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA. J. Vis. Exp. (131), e55451, doi:10.3791/55451 (2018).
Copy