A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation <em>In Vitro</em>

Zhaofeng Jia; Yujie Liang; Bin Ma; Xiao Xu; Jianyi Xiong; Li Duan; Daping Wang

doi:10.3791/55565

Dziennik

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Biologia Rozwoju

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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation <em>In Vitro</em>

JoVE Journal
Biologia Rozwoju

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JoVE Journal Biologia Rozwoju

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

16,757 Views

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10:07 min

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May 17, 2017

DOI:

10.3791/55565-v

DOI:

10.3791/55565-v

•

10:07 min

May 17, 2017

•

10 Views

Zhaofeng Jia^1,2, Yujie Liang³, Bin Ma^4,5, Xiao Xu^2,6, Jianyi Xiong², Li Duan², Daping Wang^1,2

¹Guangzhou Medical University, ²Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), ³Department of Chemistry,The Chinese University of Hong Kong, ⁴School of Biomedical Engineering,Shanghai Jiao Tong University, ⁵Renji Hospital Clinical Stem Cell Research Center,Shanghai Jiao Tong University School of Medicine, ⁶Shantou University Medical College

Transkrypcja

Podsumowanie

We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.