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December 27, 2017
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This protocol describes a method to image resident tumor-infiltrating CD8 T cells labeled with fluorescently coupled antibodies within human lung tumor slices. This technique permits real-time analyses of CD8 T cell migration using confocal microscopy.
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Peranzoni, E., Bougherara, H., Barrin, S., Mansuet-Lupo, A., Alifano, M., Damotte, D., Donnadieu, E. Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy. J. Vis. Exp. (130), e55709, doi:10.3791/55709 (2017).
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