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November 08, 2017
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In this study, we present an easy and efficient procedure for evaluating the disruption of the blood-brain barrier under neurodegenerative conditions. To achieve our objective, we infused high molecular weight fluorescein isothiocyanate labelled (FITC)-albumin into mouse jugular vein and evaluated its leakage into the brain parenchyma by fluorescence microscopy.
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Di Pardo, A., Castaldo, S., Capocci, L., Amico, E., Maglione, V. Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease. J. Vis. Exp. (129), e56389, doi:10.3791/56389 (2017).
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