11,317 Views
•
12:46 min
•
December 06, 2017
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.
Powiązane Filmy
14:21
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
Powiązane Filmy
12847 Views
11:13
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
Powiązane Filmy
8802 Views
08:18
Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
Powiązane Filmy
10644 Views
04:17
Nuclear Migration in the Drosophila Oocyte
Powiązane Filmy
3786 Views
13:59
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
Powiązane Filmy
11337 Views
07:25
Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
Powiązane Filmy
11270 Views
09:57
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
Powiązane Filmy
18245 Views
09:11
Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes
Powiązane Filmy
9025 Views
07:39
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
Powiązane Filmy
7431 Views
09:05
Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
Powiązane Filmy
302 Views
Read Article
Cytuj ten artykuł
Perkins, A. T., Bickel, S. E. Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes. J. Vis. Exp. (130), e56802, doi:10.3791/56802 (2017).
Copy