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April 27, 2018
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Here we present protocols for collecting and microinjecting precellular western corn rootworm embryos for the purpose of performing functional-genomic assays such as germline transformation and CRISPR/Cas9-genome editing.
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Chu, F., Wu, P., Pinzi, S., Grubbs, N., Lorenzen, M. D. Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing. J. Vis. Exp. (134), e57497, doi:10.3791/57497 (2018).
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