A <em>Drosophila In Vivo</em> Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

Dan Li; Feng Li; Pavithran Guttipatti; Yuanquan Song

doi:10.3791/57557

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A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

JoVE Journal
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JoVE Journal Medycyna

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

9,637 Views

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09:55 min

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May 05, 2018

DOI:

10.3791/57557-v

DOI:

10.3791/57557-v

•

09:55 min

May 05, 2018

•

3 Views

Dan Li*¹, Feng Li*¹, Pavithran Guttipatti¹, Yuanquan Song^1,2

¹Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine,University of Pennsylvania

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Podsumowanie

Here, we present a protocol using the Drosophila sensory neuron - dendritic arborization (da) neuron injury model, which combines in vivo live imaging, two-photon laser axotomy/dendriotomy, and the powerful fly genetic toolbox, as a platform for screening potential promoters and inhibitors of neuroregeneration.