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July 03, 2018
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Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.
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Benning, L., Robinson, S., Follo, M., Heger, L. A., Stallmann, D., Duerschmied, D., Bode, C., Ahrens, I., Hortmann, M. Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease. J. Vis. Exp. (137), e57950, doi:10.3791/57950 (2018).
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