Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Maria Davydova; Guillaume Dewaele Le Roi; Pierre Adumeau; Brian M. Zeglis

doi:10.3791/59063

Dziennik

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Chemia

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Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

JoVE Journal
Chemia

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JoVE Journal Chemia

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

9,310 Views

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08:47 min

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March 06, 2019

DOI:

10.3791/59063-v

DOI:

10.3791/59063-v

•

08:47 min

March 06, 2019

•

9 Views

Maria Davydova¹, Guillaume Dewaele Le Roi^1,2, Pierre Adumeau¹, Brian M. Zeglis^1,2,3,4

¹Department of Chemistry,Hunter College of the City University of New York, ²Ph.D. Program in Chemistry,Graduate Center of the City University of New York, ³Department of Radiology,Memorial Sloan Kettering Cancer Center, ⁴Department of Radiology,Weill Cornell Medical College

Transkrypcja

Podsumowanie

In this protocol, we will describe the synthesis of PODS, a phenyoxadiazolyl methyl sulfone-based reagent for the site-selective attachment of cargos to the thiols of biomolecules, particularly antibodies. In addition, we will describe the synthesis and characterization of a PODS-bearing bifunctional chelator and its conjugation to a model antibody.