Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Yongjian Zhao; Qiang Wang; Shufen Liu; Yongjun Wang; Bing Shu; Dongfeng Zhao

doi:10.3791/59171

Przygotowanie granulatu Gushukang (GSK) do eksperymentów in vivo i in vitro

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Medicine

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JoVE Journal Medicine

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Przygotowanie granulatu Gushukang (GSK) do eksperymentów in vivo i in vitro

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06:16 min

May 9, 2019

DOI: 10.3791/59171-v

Yongjian Zhao^1,2,3, Qiang Wang^1,2,3, Shufen Liu^1,2,3, Yongjun Wang^1,2,3,4, Bing Shu^1,2,3, Dongfeng Zhao^1,2,3

¹Longhua Hospital,Shanghai University of Traditional Chinese Medicine, ²Spine Institute,Shanghai University of Traditional Chinese Medicine, ³Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, ⁴School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine

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Overview

This article provides a detailed protocol for preparing a working solution of Gushukang granules for animal studies and GSK granule containing serum for in vitro experiments. This method is cost-effective and can be easily repeated, making it suitable for pharmacological investigations of herbal medicines.

Key Study Components

Area of Science

Pharmacology
Herbal Medicine
Animal Studies

Background

Preparation of granules for in vivo and in vitro studies.
Use of Gushukang granules in pharmacological research.
Cost-effective materials for experimental protocols.
Importance of reproducibility in scientific research.

Purpose of Study

To provide a protocol for preparing granule solutions.
To facilitate in vivo and in vitro experiments.
To support pharmacological investigations of herbal medicines.

Methods Used

Preparation of granules by dissolving in water.
Use of calcium carbonate tablets for serum preparation.
Intragastric administration techniques for animal studies.
Manual restraint of mice for administration.

Main Results

Successful preparation of granule solutions for experiments.
Demonstrated ease of method replication.
Establishment of a protocol for both in vivo and in vitro studies.
Cost-effective approach to pharmacological research.

Conclusions

The protocol is effective for preparing Gushukang granules.
Supports further research in herbal medicine pharmacology.
Encourages reproducibility and accessibility in scientific studies.

Frequently Asked Questions

What are Gushukang granules?

Gushukang granules are a type of herbal medicine used in various pharmacological studies.

How are the granules prepared for experiments?

The granules are dissolved in water and combined with calcium carbonate for serum preparation.

What is the significance of this protocol?

It provides a cost-effective and reproducible method for conducting in vivo and in vitro studies.

Can this method be used for other herbal medicines?

Yes, the protocol can be adapted for various herbal medicines in pharmacological research.

What type of animals are used in this study?

The protocol specifically mentions the use of C57 Black 6 mice for in vivo experiments.

Is this method suitable for large-scale studies?

Yes, the low cost and ease of replication make it suitable for larger studies.

Ten artykuł zawiera szczegółowy protokół przygotowania roztworu roboczego granulatu Gushukang do badań na zwierzętach oraz granulatu GSK zawierającego surowicę do eksperymentów in vitro. Protokół ten może być stosowany do badań farmakologicznych leków ziołowych, a także do recept zarówno na eksperymenty in vivo, jak i in vitro.

Protokół ten umożliwia przygotowanie roztworu roboczego granulek do badań in vivo, a także przygotowanie surowicy zawierającej granulki do eksperymentów in vitro. Jedną z zalet tej techniki jest to, że metodę można łatwo powtórzyć ze względu na niski koszt materiałów i zwierząt, które tworzysz do eksperymentów. Zacznij od dodania ośmiu, 16 i 24 gramów granulek Gushukang lub GSK do pojedynczych 50-mililitrowych probówek.

Dodaj 48 mililitrów wody do każdej probówki i potrząśnij probówkami, aby całkowicie rozpuścić granulki. Następnie umieść 1,6 tabletki węglanu wapnia uzupełnionego witaminą D3 w innej 50-mililitrowej probówce i rozpuść tabletki w 48 mililitrach soli fizjologicznej, wstrząsając. W przypadku dożołądkowego podawania roztworów, najpierw delikatnie, ale stanowczo ręcznie należy powstrzymać jednomiesięczną samicę myszy C57 Black 6 przez dwie do trzech minut.

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