Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Carl  Robert Rankin; Janet Treger; Emmanuelle Faure-Kumar; Jihane Benhammou; Deborah Anisman-Posner; Alex  Edward Bollinger; Charalabos Pothoulakis; David Miguel Padua

doi:10.3791/59233

Dziennik

/

Genetyka

/

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

JoVE Journal
Genetyka

Aby wyświetlić tę treść, wymagana jest subskrypcja JoVE. Zaloguj się lub rozpocznij bezpłatny okres próbny.

JoVE Journal Genetyka

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

8,834 Views

•

13:04 min

•

March 01, 2019

DOI:

10.3791/59233-v

DOI:

10.3791/59233-v

•

13:04 min

March 01, 2019

•

8824 Views

Carl Robert Rankin¹, Janet Treger¹, Emmanuelle Faure-Kumar², Jihane Benhammou¹, Deborah Anisman-Posner³, Alex Edward Bollinger³, Charalabos Pothoulakis¹, David Miguel Padua¹

¹Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine,University of California, Los Angeles ²Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine,University of California, Los Angeles ³Division of Hematology and Oncology, Department of Medicine,University of California, Los Angeles

Podsumowanie

Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.