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April 05, 2019
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This protocol describes the use of high-specificity ion-exchange chromatography with multi-angle light scattering for an accurate molar mass determination of proteins, protein complexes, and peptides in a heterogeneous sample. This method is valuable for quality assessment, as well as for the characterization of native oligomers, charge variants, and mixed-protein samples.
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Amartely, H., Some, D., Tsadok, A., Lebendiker, M. Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization. J. Vis. Exp. (146), e59408, doi:10.3791/59408 (2019).
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