Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Lesheng Zhang; Tom Cambron; Yueqing Niu; Zigang Xu; Ning Su; Hongyan Zheng; Karl Wei; Paula Ray

doi:10.3791/60186

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Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

JoVE Journal
Chemia

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JoVE Journal Chemia

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

7,091 Views

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09:32 min

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September 26, 2019

DOI:

10.3791/60186-v

DOI:

10.3791/60186-v

•

09:32 min

September 26, 2019

•

6 Views

Lesheng Zhang*¹, Tom Cambron*², Yueqing Niu¹, Zigang Xu³, Ning Su⁴, Hongyan Zheng⁴, Karl Wei², Paula Ray²

¹Procter and Gamble,Beijing Innovative center, ²Procter and Gamble,Mason Business Center, ³Department of Dermatology,Beijing Children’s Hospital, ⁴Chinese Academy of Inspection and Quarantine

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Here, we present a protocol for collection of confocal Raman spectra from human subjects in clinical studies combined with chemometric approaches for spectral outlier removal and the subsequent extraction of key features.

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Raman Spectra Stratum Corneum Chemometrics Skin Analysis Water Measurement Protein Measurement Lipid Measurement Spectroscopic Artifacts Outlier Removal Data Processing Imaging Window MATLAB Software Baseline Correction Automatic Weighted Least Squares Data Preprocessing Clinical Data Set

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Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

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