The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Elaine  M. Mihelc; Stephanie Angel; Robert  V. Stahelin; Seema Mattoo

doi:10.3791/60677

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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

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Biologia

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JoVE Journal Biologia

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

9,461 Views

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11:45 min

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February 27, 2020

DOI:

10.3791/60677-v

DOI:

10.3791/60677-v

•

11:45 min

February 27, 2020

•

1 Views

Elaine M. Mihelc¹, Stephanie Angel², Robert V. Stahelin^2,3,4, Seema Mattoo^1,4,5,6

¹Department of Biological Sciences,Purdue University, ²Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University, ³Purdue Institute of Inflammation, Immunology and Infectious Disease,Purdue University, ⁴Bindley Biosciences Center,Purdue University, ⁵Purdue Institute of Integrative Neuroscience,Purdue University, ⁶Purdue Center for Cancer Research,Purdue University

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This protocol describes the cryoAPEX method, in which an APEX2-tagged membrane protein can be localized by transmission electron microscopy within optimally-preserved cell ultrastructure.

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CryoAPEX Method Electron Microscopy Membrane Protein Localization Cellular Preservation APEX2 Cryofixation Freeze Substitution Protein Localization Viral Replication HEK293 Cells Transfection Glutaraldehyde Sodium Cacodylate Buffer DAB Solution

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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

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