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March 11, 2020
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The mammary gland is a bilayered structure, comprising outer myoepithelial and inner luminal epithelial cells. Presented is a protocol to prepare organoids using differential trypsinization. This efficient method allows researchers to separately manipulate these two cell types to explore questions concerning their roles in mammary gland form and function.
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Rubio, S., Cazares, O., Macias, H., Hinck, L. Generation of Mosaic Mammary Organoids by Differential Trypsinization. J. Vis. Exp. (157), e60742, doi:10.3791/60742 (2020).
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