Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

Dmitry Frank; Benjamin  F. Gruenbaum; Julia Grinshpun; Israel Melamed; Olena Severynovska; Ruslan Kuts; Michael Semyonov; Evgeni Brotfain; Alexander Zlotnik; Matthew Boyko

doi:10.3791/61309

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Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

JoVE Journal
Neurologia

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JoVE Journal Neurologia

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

7,800 Views

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04:32 min

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October 23, 2020

DOI:

10.3791/61309-v

DOI:

10.3791/61309-v

•

04:32 min

October 23, 2020

•

5 Views

Dmitry Frank*¹, Benjamin F. Gruenbaum*², Julia Grinshpun¹, Israel Melamed³, Olena Severynovska⁴, Ruslan Kuts¹, Michael Semyonov¹, Evgeni Brotfain¹, Alexander Zlotnik¹, Matthew Boyko¹

¹Department of Anesthesiology and Critical Care, Soroka Medical Center,Ben-Gurion University of the Negev, ²Department of Anesthesiology,Yale University School of Medicine, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine,Dnepropetrovsk State University

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This protocol describes a novel technique of measuring the three most important parameters of ischemic brain injury on the same set of rodent brain samples. Using only one brain sample is highly advantageous in terms of ethical and economic costs.

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Post-stroke Cerebral Edema Infarct Zone Blood-brain Barrier Ischemic Brain Injury Rodent Brain Samples Middle Cerebral Artery Occlusion BBB Permeability Image Analysis Macro Infarct Volume Trichloroacetic Acid Luminescence Spectrophotometry

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Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

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