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November 20, 2021
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This protocol presents steps to acquire and analyze fluorescent calcium images from brain ensheathing pericytes and blood flow data from nearby blood vessels in anesthetized mice. These techniques are useful for studies of mural cell physiology and can be adapted to investigate calcium transients in any cell type.
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Meza-Resillas, J., Ahmadpour, N., Stobart, M., Stobart, J. Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo. J. Vis. Exp. (177), e62725, doi:10.3791/62725 (2021).
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