Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Rasmus Jensen; Niels &#216;rtenblad; Cristiano di Benedetto; Klaus Qvortrup; Joachim Nielsen

doi:10.3791/63347

Dziennik

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Biochemia

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Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

JoVE Journal
Biochemia

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JoVE Journal Biochemia

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

3,925 Views

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08:32 min

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February 07, 2022

DOI:

10.3791/63347-v

DOI:

10.3791/63347-v

•

08:32 min

February 07, 2022

•

9 Views

Rasmus Jensen¹, Niels Ørtenblad², Cristiano di Benedetto³, Klaus Qvortrup³, Joachim Nielsen²

¹Research center for applied health science,University College South Denmark, ²Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, ³Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Transkrypcja

Podsumowanie

A modified post-fixation procedure increases the contrast of glycogen particles in tissue. This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle.