Ekspresja transgenu wirusa w sercach gryzoni i ocena ryzyka arytmii serca

Overview

This protocol describes a minimally invasive technique for achieving efficient transgene expression in the hearts of rats and mice using direct intramyocardial injection of a virus under ultrasound guidance. The protocol also includes methods to assess ventricular arrhythmia susceptibility via programmed electrical stimulation in isolated, Langendorff-perfused hearts.

Key Study Components

Research Area

• Cardiac arrhythmias
• Ventricular function
• Gene expression studies in animal models

Background

• Importance of studying cardiac arrhythmias
• Existing conventional approaches involve greater invasiveness
• Potential for using small animal models to study heart disease

Methods Used

• Intramyocardial injection of viral vectors
• Mice and rats as model organisms
• Langendorff perfusion system and ECG monitoring

Main Results

• Injection technique demonstrated effective transgene expression
• Increased susceptibility to arrhythmias in myocardial infarction models
• Protocol validated by contrasting results in healthy vs. affected hearts

Conclusions

• This study provides an efficient methodology for gene expression analysis in cardiac tissues
• The results contribute to understanding arrhythmia mechanisms in heart disease research

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