Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Ying Wang; Zhuo An; Zhifang Zhao; Can Li; Jiangbo Fan

doi:10.3791/64776

Dziennik

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Biologia

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Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

JoVE Journal
Biologia

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JoVE Journal Biologia

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

3,483 Views

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05:44 min

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November 25, 2022

DOI:

10.3791/64776-v

DOI:

10.3791/64776-v

•

05:44 min

November 25, 2022

•

24 Views

Ying Wang^1,2, Zhuo An^1,2, Zhifang Zhao^1,2, Can Li³, Jiangbo Fan^1,2

¹Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology,Shanghai Jiao Tong University, ²Joint Center for Single Cell Biology, School of Agriculture and Biology,Shanghai Jiao Tong University, ³Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education),Shanghai Jiao Tong University

Transkrypcja

Podsumowanie

Here, we describe a method for the real-time detection of apoplastic reactive oxygen species (ROS) production in rice tissues in pathogen-associated molecular pattern-triggered immune response. This method is simple, standardized, and generates highly reproducible results under controlled conditions.