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June 30, 2023
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This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.
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Cheng, J., Zhu, J., Liu, Y., Yang, C., Zhang, Y., Liu, Y., Jin, C., Wang, J. Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages. J. Vis. Exp. (196), e65521, doi:10.3791/65521 (2023).
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