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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

November 3rd, 2023
*1, *1, 1, 2, 3, 2, 2, 2, 3, 1,2,4, 2
1Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, 3Department of Biomedical Engineering, University of Miami, 4VA Tennessee Valley Healthcare System
* These authors contributed equally

Summary
Automatic Translation

Detta protokoll beskriver en metod för synkron insamling och samregistrering av intracellulära signaleringshändelser och utsöndring av insulin och glukagon av primära humana pseudoöar med hjälp av adenoviral tillförsel av en cyklisk adenosinmonofosfat (cAMP) biosensor, en cAMP-differensdetektor in situ (cADDis) och ett mikroperifusionssystem.

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