Question 1

What is the Hayflick limit and why do normal cells stop dividing?

Accepted Answer

The Hayflick limit is the maximum number of times normal cells can divide, typically 40-60 divisions for fetal cells. This limit occurs because telomeres—protective caps on chromosomes—shorten with each cell division. When telomeres become too short, cells enter replicative senescence and can no longer divide, preventing unlimited proliferation.

Question 2

How are cell lines created from normal cells?

Accepted Answer

Scientists create immortalized cell lines by chemically or genetically manipulating normal cells to bypass the Hayflick limit. One key method involves overexpressing telomerase, an enzyme that prevents telomere shortening during cell division. This allows cells to proliferate indefinitely while retaining characteristics of their tissue of origin.

Question 3

What are the differences between primary and transformed cell lines?

Accepted Answer

Primary cell lines retain the genotypic and phenotypic characteristics of their original tissue, with normal chromosome numbers like 46 in human cells. Transformed cell lines derived from cancer cells show anchorage independence, lack contact inhibition, and often have altered chromosome numbers—for example, SW480 cells have up to 56 chromosomes and A549 cells up to 66.

Question 4

Why are cell lines prone to contamination and genetic changes?

Accepted Answer

Cell lines undergo phenotypic and genetic changes over time due to genomic instability and environmental stress in laboratory conditions. They are also susceptible to cross-contamination from other cell cultures in the lab. These issues make routine validation essential to ensure cell line authenticity and reliability for research applications.

Question 5

What methods are used to validate cell lines?

Accepted Answer

Spectral karyotyping identifies numerical and structural chromosomal aberrations and detects cross-contamination by visualizing chromosome structure. STR profiling analyzes short tandem repeats in DNA, which are unique to each cell line, providing molecular-level validation. These techniques ensure cell lines remain authentic and uncontaminated throughout research use.

Question 6

What are the practical advantages of using cell lines in research?

Accepted Answer

Cell lines are easy to handle, cost-effective, and bypass ethical issues associated with animal research. They enable researchers to model diseases, test drugs, and mass-produce antibodies and vaccines efficiently. Despite limitations like genetic drift and contamination risk, their accessibility and scalability make them invaluable for routine laboratory applications.

Question 7

How can cancerous cell lines be generated in the laboratory?

Accepted Answer

Primary cell lines can acquire spontaneous mutations in cancer-associated genes or be deliberately exposed to cancer-inducing viruses or chemicals to create transformed cell lines. These cancerous cell lines then exhibit properties like unlimited proliferation, anchorage independence, and altered chromosome numbers, making them useful models for studying cancer biology and drug responses.

Linhas Celulares

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