Antibody-Dependent Enhancement of Zika Virus by Dengue Antibodies in Human Primary Macrophages

Resumo

Source: Asad, S., et al. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (2019)

This video demonstrates antibody-dependent enhancement of Zika virus infection in human primary macrophages, using dengue antibody–virus complexes to model how cross-reactive antibodies facilitate Fcγ receptor–mediated viral entry and replication for studying infection mechanisms and immune interactions.

Protocolo

NOTE: This protocol can be used to examine ADE (antibody-dependent enhancement) of ZIKV (Zika virus) replication in any human cell type that expresses the Fcγ receptor. The protocol consists of three parts (Figure 1).

1. Cell Seeding and Infection Setup:
NOTE: For this particular study, human primary macrophages or U937 myelomonocytic cell line (ATCC-CRL-1593.2) were used. The cells were maintained in Roswell Park Memorial Institute (RPMI) growth medium supplemented with 10% fetal bovine serum (FBS) at a 37 °C incubator with 5% carbon dioxide (CO₂). All the steps were carried out in a bios....

Resultados

Figure 1: Diagrammatic illustration of the ADE protocol. A step-by-step illustration shows all the steps involved in the whole protocol.

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Materiais

Lista de materiais utilizados neste artigo

Nome	Empresa	Número de catálogo	Comentários
Fetal Bovine Serum	GEMINI	100-106
RPMI-medium	Gibco	11875093