Spheroid Microplate
Spheroid Microplate - Related Videos
07:51
Introduction to the Microplate Reader
0 Views •
2023
The microplate reader is a multimodal instrument that allows for a variety of experiments to be performed and measured simultaneously. Microplate readers can make absorbance, fluorescence and luminescence measurements. Multiwell plates are integral to the microplate reader and allow for many experiments to be performed at once. Regardless of the assay type, experiments on the plate reader utilize a standard curve to determine the experimental values. This curve uses samples of known...
03:18
Generation of a Three-Dimensional Spheroid Model of Tumor Cell Invasion
0 Views •
2025
Source: Guyon, J., et al. A 3D Spheroid Model for Glioblastoma. J. Vis. Exp. (2020)The video demonstrates the generation of a three-dimensional spheroid model of tumor cell invasion. Spheroids are formed from human brain tumor cells and embedded in a collagen matrix. The tumor cells at the spheroid periphery adopt an invasive phenotype, secreting proteases to degrade the collagen and advancing to invade the surrounding matrix.
03:20
Splitting Spheroids for Sub-culturing and Shipping: A Procedure for Propagating and Transferring Spheroids from Small Bowel Neuroendocrine Tumor
0 Views •
2023
This video demonstrates a strategy to split and propagate spheroids developed from small bowel neuroendocrine tumor cells. These spheroids have small bowel neuroendocrine tumor markers and can be propagated and further used to test anti-cancer drugs.
04:29
Immunofluorescent Staining of Spheroids: A Technique to Analyze Spheroids for Expression of Intra- and Extracellular Antigen Expression
0 Views •
2023
This video describes the technique of immunofluorescent staining of spheroids to understand the differentiation of the fibroblasts or CAFs. This protocol helps detect expression of intra- and extracellular antigens, including deposition of ECM proteins within the spheroids.
02:59
Embedding 3D Spheroids in Agarose Gel Drops: A Technique to Preserve Spheroids for Downstream Analysis
0 Views •
2023
This video describes the technique to embed 3D spheroids in agarose gel drops for preservation. The embedded 3D spheroids can be further used for downstream analysis.
FAQs
Nonadherent well surfaces limit attachment to the plate, encouraging cells to remain together and form aggregates through cell-cell adhesion. The often round-bottom geometry helps concentrate cells into a localized structure, supporting the development of relatively organized spheroids rather than dispersed growth. This configuration is central to generating reproducible three-dimensional cultures.
As spheroids become three-dimensional and densely organized, nutrients and oxygen must move from the surrounding culture medium toward the interior. This produces gradients across the aggregate, with the core receiving less access than outer regions. These spatial differences create tissue-like conditions that can influence cellular behavior and treatment responses within the same model.
A Spheroid Microplate supports cell organization in three dimensions, whereas conventional two-dimensional culture grows cells on a flat surface. The three-dimensional arrangement permits cell-cell interactions, internal nutrient and oxygen gradients, and reduced drug penetration toward the core. Consequently, it can provide a more physiologically relevant setting for studying solid-tumor biology and therapeutic response.
Standardized well geometry helps create spheroids with more consistent formation conditions from well to well. Greater uniformity can make comparisons between treatments easier because differences in aggregate structure are less likely to dominate the experiment. In cancer research, this supports controlled evaluation of tumor behavior and responses across multiple culture conditions.
Maintenance depends on using wells that support nonadherent growth and on keeping culture conditions controlled throughout the experiment. The plate design should allow cells to remain aggregated while preserving the three-dimensional structure over time. Consistent conditions are important because changes in aggregate organization can alter gradients, drug access, and the interpretation of treatment responses.
Researchers can use these plates when they need treatment models that reflect features of solid tumors more closely than flat cultures. Spheroids allow assessment of how therapies perform when cell-cell interactions, internal gradients, and limited penetration toward the core influence exposure. This makes the system useful for therapeutic screening and comparison of treatment responses.
These cultures can support studies of tumor biology by reproducing spatial and cellular features associated with solid tumors. Investigators can examine how three-dimensional organization affects cell interactions, access to nutrients and oxygen, and movement of treatments into the aggregate. The resulting model provides a controlled in vitro system for evaluating cancer-related responses under tissue-like conditions.