Four-chamber Dissection

Four-chamber dissection is an anatomical method that opens the right and left atria and ventricles of a four-chambered heart to reveal internal structures and spatial relationships. By making carefully positioned incisions and separating the cardiac walls, the procedure exposes the atrioventricular and semilunar valves, septa, major vessels, and pathways through which blood moves from the atria to the ventricles and then into the pulmonary or systemic circulation. In medicine, this hands-on approach supports learning cardiac anatomy, correlating structure with circulation, and interpreting abnormalities relevant to clinical examination, imaging, surgery, and pathology.

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JoVE Journal - Developmental Biology

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The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.

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JoVE Journal - Biology
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The Drosophila egg chamber is an excellent model for studying the mechanisms of mRNA localization. In order to capture the dynamic events that underpin the processes of localization, rapid high resolution imaging of live tissue is required. Here, we present a protocol for dissection and imaging of live samples with minimal disruption.

Education

JoVE Core - Anatomy and Physiology

Chambers of the Heart

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2025

The human heart is a complex organ made up of four chambers: the right and left atria and the right and left ventricles. These internal chambers are separated by partitions known as the interatrial and interventricular septa. The exterior of the heart features a groove known as the coronary sulcus that demarcates the atria from the ventricles, while the anterior and posterior interventricular sulci distinguish between the two ventricles. Deoxygenated blood from the body is received in the right...

A Customizable Chamber for Measuring Cell Migration

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Cited by 2 •

2017

This protocol details a customizable method to measure cell migration in response to chemoattractants that may also be used to determine the diffusion rate of a drug out of a polymer matrix.

Research

JoVE Journal - Biology
Free Sample

Dissection of Organs from the Adult Zebrafish

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Cited by 168 •

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This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.

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