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A Customizable Chamber for Measuring Cell Migration
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JoVE Journal Biology
A Customizable Chamber for Measuring Cell Migration

A Customizable Chamber for Measuring Cell Migration

DOI:
10.3791/55264-v

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07:33 min

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March 12, 2017

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, , , , , , ,
1Department of Bioengineering,Clemson University

Chapters

  • 00:05Title
  • 00:39Production of an Acrylic Mold of the Microchannel Chamber
  • 01:37Formation of a Microchannel Chamber
  • 02:28Sterilization of the Microchannel Chamber
  • 03:15Cell Seeding in the Microchannel Chamber
  • 04:18Formation and Chemotactic Factor Loading of an Agarose Block
  • 05:19Time-lapse Imaging of Cell Migration
  • 05:43Results: Time-lapse Cell Migration Tracking
  • 06:14Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol details a customizable method to measure cell migration in response to chemoattractants that may also be used to determine the diffusion rate of a drug out of a polymer matrix.

Tags

Keywords: Cell MigrationChemotacticChemorepulsiveWound HealingMicro-channelCADAcrylicLaser CuttingPDMSElastomerVacuumUV SterilizationCell Culture

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