Tumor Tissue Collection
Tumor Tissue Collection - Related Videos
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
02:31
FFPE Tumor Tissue Macrodissection: A Technique to Obtain Specific Tumorous Tissue from Unstained Specimens
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2023
This video describes a method for the manual macrodissection of cancer tissue from unstained tissue specimens to obtain a specific cancer tissue. The tissue section can be processed further to obtain DNA for genome-wide studies on gastrointestinal cancers.
08:23
Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection
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Cited by 1 •
2022
The present protocol describes a standardized resection of brain tumors in rodents through a minimally invasive approach with an integrated tissue preservation system. This technique has implications for accurately mirroring the standard of care in rodent and other animal models.
05:33
Lung-tumor Tissue Explant Culture: A Procedure to Generate and Culture Precision-cut Lung Tumor Slices
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2023
Organotypic primary tissue explant cultures, which include precision-cut slices, represent the three-dimensional (3-D) tissue architecture as well as the multicellular interactions of native tissue. This video describes the culturing of murine lung tumor-derived tissue slices using a rotating incubation unit, a system that enables intermittent exposure of tissues to oxygen and nutrients. The protocol describes the methodology to generate and culture precision-cut lung tumor slices for drug...
09:33
Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
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2026
This protocol successfully prepared sufficient high-quality neovascular tissue from glioma samples using laser-capture microdissection for quantitative proteomics analysis of tumor angiogenesis, overcoming the limitations of studies that traditionally focus mainly on angiogenic factors and providing a large-scale proteomics profile of glioma angiogenesis.
FAQs
Maintaining cellular structure and molecular information is central because the specimen must remain suitable for multiple forms of analysis. Histology depends on preserved tissue architecture, while immunohistochemistry and molecular testing require relevant biological information to remain interpretable. Collection conditions are therefore applied to protect specimen integrity from acquisition through downstream examination.
These methods answer complementary questions. Histology evaluates the tissue’s cellular and structural features, immunohistochemistry assesses relevant markers in the tissue, and molecular testing examines molecular information. Together, they support tumor classification and biomarker identification rather than relying on a single type of evidence. This combined analysis can inform diagnosis and treatment planning.
Labeling and transport preserve the connection between a specimen and the clinical or research question it is meant to address. Correct labeling supports reliable interpretation of findings, while suitable transport conditions help maintain cellular structure, molecular information, and overall integrity. These controls make the collected material more useful for diagnosis, profiling, and research.
A controlled workflow begins with acquisition through surgical resection or biopsy. The specimen is then preserved, labeled, and transported under conditions intended to maintain cellular structure, molecular information, and integrity. After receipt, pathologists can apply histology, immunohistochemistry, and molecular testing. Coordinating these stages supports dependable interpretation of the collected material.
The immediate clinical purposes are diagnosis and treatment planning. Collected tissue allows pathologists to classify the tumor and examine clinically relevant biomarkers using histology, immunohistochemistry, and molecular testing. Those findings can contribute to tumor profiling and help support personalized medicine, in which treatment planning considers information obtained from the individual tumor.
Personalized medicine depends on information that distinguishes one tumor from another. Tissue analysis can provide classification data and identify clinically relevant biomarkers through histology, immunohistochemistry, and molecular testing. These results contribute to tumor profiling, giving clinicians evidence that can be incorporated into treatment planning rather than relying only on broad tumor categories.
Collected specimens provide research material for studying cancer biology and evaluating potential therapies. Their preserved cellular structure and molecular information allow investigators to examine features of tumors using tissue-based and molecular analyses. This research context extends the value of collection beyond diagnosis, linking specimen integrity with efforts to understand disease and assess possible treatments.