Parafilm Mold Droplets

Parafilm mold droplets are a simple microscale technique for forming and containing discrete aqueous compartments, enabling controlled handling of small sample volumes. A shaped sheet of hydrophobic Parafilm confines liquid by limiting spreading, while surface tension maintains each droplet as an isolated compartment during preparation or incubation. In neuroscience, these temporary molds can organize media, reagents, or biological samples for localized assays, cell handling, and tissue-related experiments. Their low cost, flexibility, and straightforward setup support reproducible small-volume workflows while reducing reagent use and helping researchers perform parallel manipulations under controlled conditions.

Parafilm Mold Droplets - Related Videos

Research

JoVE Journal - Bioengineering

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

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Cited by 34 •

2014

We have devised a method for low-cost and rapid prototyping of liquid elastomer rubber injection molded devices by using fused deposition modeling 3D printers for mold design and a modified desiccator as a liquid injection system.

Production and Use of Customizable Agarose Molds for Scaffold-Free Mouse Ovarian Follicle Culture

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Cited by 2 •

2025

We describe methods to culture ovarian follicles in a novel scaffold-free agarose mold that complement in vitro gametogenesis.

Research

JoVE Journal - Biology
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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

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Cited by 19 •

2016

The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Cited by 18 •

2016

Multicolor fluorescence detection in droplet microfluidics typically involves bulky and complex epifluorescence microscope-based detection systems. Here we describe a compact and modular multicolor detection scheme that utilizes an array of optical fibers to temporally encode multicolor data collected by a single photodetector.

Acrylic Resin Molding Based Head Fixation Technique in Rodents

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Cited by 4 •

2016

Traditional head fixation techniques have used metallic frames attached on the skull as an interface for head fixation apparatus. This protocol demonstrates a frameless, acrylic resin molding based head fixation technique for behavioral tasks in rodents.

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