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The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
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The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

Article DOI: 10.3791/1206-v • 08:58 min • March 13th, 2009
March 13th, 2009 •
1, 1, 1
1Department of Biology, University of Waterloo

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A simple, inexpensive, and effective method of preparing Drosophila embryos for live-imaging analysis is presented. Our protocol provides humidity and gas exchange and does not compress the Drosophila embryo. This method is suitable for GFP-based live imaging of Drosophila embryos using a stereomicroscope or upright compound microscope.

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Drosophila Embryos Live-imaging Hanging Drop Protocol GFP-based Timelapse Genetic Regulation Tissue Morphogenesis Cell-cell Adhesion Cell Death Microscopy Dehydration Hypoxia Halocarbon Oil Gas-permeable Membrane Coverslip Compression Biomechanical-based Analysis Viability Halocarbon Oil Humid Chamber Gas Exchange
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