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Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
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Biologia
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JoVE Journal Biologia
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

DOI:
10.3791/1350-v

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07:47 min

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August 14, 2009

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,
1Protein Expression and Purification Core Facility,EMBL Heidelberg

Capítulos

  • 00:05Título
  • 01:02Introduction
  • 01:34Preparation of the CBB Staining Solution
  • 02:38SDS-PAGE
  • 04:26Staining of the Gel
  • 06:38Representative Results / Outcome
  • 07:19Conclusion

Summary

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A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.

Tags

ProteinsGelsCoomassie G-250StainingOrganic SolventAcetic AcidSDS-PAGEMicrowave OvenToxic SolventsSafety ConsiderationsAlternative CompositionBidistilled WaterHClWashingHeating GelProtein Staining

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