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Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE

Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

Article DOI: 10.3791/200119-v • 04:44 min • June 2nd, 2023
June 2nd, 2023 •
1, 1, 1, 4, 1, 2,3, 4, 1,5
1Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO, Université Catholique de Louvain (UCLouvain), 2Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU), Université Catholique de Louvain (UCLouvain), 3Division of Pneumology, Cliniques Universitaires St-Luc, Université Catholique de Louvain (UCLouvain), 4IREC Imaging Platform, Université Catholique de Louvain (UCLouvain), 5Institut Roi Albert II, Department of Medical Oncology and Gastroenterology, Cliniques Universitaires St-Luc

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Multiplex Immunofluorescence Spatial Image Analysis Clinical Assessment Biological Assessment Tumor Microenvironment Human Colorectal Cancer Metastatic Disease Treatment Response Predictive Biomarkers Prognostic Biomarkers Tumor Immune Microenvironment Intratumoral Microbiome RNA Sequencing Whole Exome Sequencing TCR Sequencing Circulating Cell-free DNA Analysis Immunofluorescence Staining Fluorescence Institute Hybridization Tyramide Signal Amplification Microbiome Immune Cell Interaction Robust Method Reproducible Method Cost-effective Method Tumor Microenvironment Composition
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