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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

Article DOI: 10.3791/200322-v • 03:38 min • July 28th, 2023
July 28th, 2023 •
1, 1, 1, 1, 1
1Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Centre Basel

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Biology Single Nucleus Sequencing Single-cell RNA Sequencing Single-nucleus RNA Sequencing Gene Expression Difficult-to-dissociate Tissues Archival Material Robotic Dissociator Tissue Homogenization Chemical Gradient Nuclei Filtering Automated Fluorescent Cell Counter Mouse Brain Rat Kidney Cynomolgus Liver Spleen Tissue
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