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Transferring Grids to a Perforated Tray Under DI Water, Transferring Graphene to the Grids, Removing MMA, and Cleaning the Grids (Video) | JoVE

Coating TEM Grids with a Graphene Monolayer

Article DOI: 10.3791/200453-v • 03:24 min • September 8th, 2023
September 8th, 2023 •
*1, *1, 1, 1
1Department of Structural and Computational Biology, Scripps Research
* These authors contributed equally

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Fabrication Monolayer Graphene-coated Grids Cryoelectron Microscopy Sample Preparation Vitreous Ice Fenestrated Support Film Hydrophobic Effect Denaturation Aggregation Complex Dissociation Hydrophobic Interactions 3D Reconstructions Anisotropic Directional Resolution Graphene Supports Background Noise Protein Concentration CryoEM Imaging Expense Large-scale Production
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