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JoVE Journal Genetics
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Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages

A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

Article DOI: 10.3791/200896-v • 04:03 min • August 4th, 2023
August 4th, 2023 •
1, 1, 1,2
1Department of Internal Medicine, University of California, Davis, 2Department of Medical Microbiology and Immunology, University of California, Davis

Tags

Electroporation Cas9-sgRNA Complexes CRISPR/Cas9 Genome Editing Frameshift Mutations Small Insertions And Deletions (indels) Gene Function Single-guide RNAs (sgRNA) Ribonucleoprotein Complexes (RNPs) Editing Efficiency Sanger Sequencing Analysis Program
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