Experiment
JoVE Encyclopedia of Experiments
Encyclopedia of Experiments: Biological Techniques
Encyclopedia of Experiments: Biological Techniques
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Safranin-O Staining
Suggested Video
Safranin-O Staining - ConceptTranscript
To perform Safranin-O staining, first deparaffinize the slides in oxylene for three to five minutes, repeating the oxylene bath twice. Then, hydrate the slides in an ethanol gradient, ending with distilled water. Then, place the slides through Weigert's Iron Hematoxylin solution, a 0.05% Fast Green solution, a 1% acetic acid solution, and a 0.1% Safranin-O solution. Now, mount the slides using a resinous medium.
