Journal
/
Neurociência
/
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
JoVE Journal
Neurociência
Author Produced
This content is Free Access.
JoVE Journal Neurociência
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

DOI:
10.3791/3633-v

•

09:24 min

•

October 30, 2011

•

, , , , ,
1Department of Neurosurgery,University of Florida , 2Department of Anatomical Sciences,Shiraz University of Medical Sciences

Capítulos

  • 00:05Título
  • 00:26Introduction
  • 00:49Primary GBM Neurosphere Culture
  • 05:06Passaging GBM-Derived Neurospheres
  • 07:43Representative Results
  • 08:41Conclusion

Summary

Tadução automática

Tadução automática

This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.

Tags

IsolationExpansionHuman Glioblastoma MultiformeTumor CellsNeurosphere AssayStem-like CellsBreastLungColonProstateBrainTumor Initiating Cell Population(s)Tumor FormationProgressionRecurrenceTherapeutic ApproachesNeurosphere Culture MethodChemical DigestionMechanical DissociationSingle Cell SuspensionNSA CulturePrimary Neurospheres

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Isolation and Culture of Human Fungiform Taste Papillae Cells

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Shuttle Box Assay as an Associative Learning Tool for Cognitive Assessment in Learning and Memory Studies using Adult Zebrafish

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Read Article