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DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

Article DOI: 10.3791/3667-v • 09:14 min • December 13th, 2011
December 13th, 2011 •
1, 1
1Developmental Neurobiology, Max Delbrück Center for Molecular Medicine

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The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.

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DiI-labeling DRG Neurons Axonal Branching Whole Mount Preparation Mouse Embryonic Spinal Cord Diffusive Staining Lipophilic Tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine Perchlorate (DiI) Axonal Pathways Wild-type Mutated Mouse Lines Functional Role Candidate Proteins Control Of Axonal Branching Wiring Of The Nervous System Parallel Processing Of Information Intermediate Target Regions Terminal Arborization Axonal Growth Growth Cone Splitting Branching Delayed Branching Interstitial Branching Central Projections Of Neurons Dorsal Root Entry Zone (DREZ) Stereotyped Pattern
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