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Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
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JoVE Journal Medicina
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

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10,661 Views

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11:29 min

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October 03, 2012

DOI:

10.3791/3788-v

DOI:
10.3791/3788-v

•

11:29 min
October 03, 2012

•

4 Views
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1Department of Pathology, National Prion Disease Pathology Surveillance Center,Case Western Reserve University School of Medicine, 2Department of Neurology, National Prion Disease Pathology Surveillance Center,Case Western Reserve University School of Medicine

Summary

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A new species of cellular prion protein (PrPC) has recently been identified in uninfected human brains using the methods described here. These methods can be used to isolate various PrP species, while some of them are also useful in isolating other misfolded protein aggregates from human brains.

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Cite this Article

Xiao, X., Yuan, J., Zou, W. Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain. J. Vis. Exp. (68), e3788, doi:10.3791/3788 (2012).

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