Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Flow Cytometric Isolation of Primary Murine Type…
JoVE Journal
Immunology and Infection

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Immunology and Infection
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections (Video) | JoVE
Journal (Immunology and Infection)
Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections (Video) | JoVE
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Journal (Immunology and Infection)
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
 

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Article DOI: 10.3791/4322-v • 14:48 min • December 26th, 2012
December 26th, 2012 •
1,2, 1,2, 3, 2, 1,2, 1
1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research

Capítulos

Resumo
Automatic Translation

We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.

Tags

Flow Cytometric Isolation Primary Murine Type II Alveolar Epithelial Cells Functional Studies Molecular Studies Immune Regulation Cytokine Production Antigen-presenting Cells Infection T-cell Mediated Autoimmunity Clinical Contexts Airway Hyper-reactivity Infections Cell Lines Large Numbers Of Cells Complex Processes Autoimmune Inflammation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter