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Neuroscience

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JoVE Journal Neuroscience
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Article DOI: 10.3791/50146-v • 08:44 min • September 24th, 2013
September 24th, 2013 •
1, 2, 2, 3, 1
1Department of Neuropathology, Medical School Düsseldorf, 2Department of Molecular Genetics, Weizmann Institute for Science, 3Center of Behavioral Neuroscience, University of Düsseldorf

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Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.

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In Utero Electroporation Genetic Modification Neuronal Development Behavior Investigation Neuropathology Monitoring Transfection Success Non-invasive Techniques Postnatal Animals E16 Rats Nucleic Acids Tweezer Electrodes Developing Cortex Hippocampus Luciferase Gene Transfected Cells In Vivo Bioluminescence IVIS Spectrum Device Quantification Software Area Definition Cortical Electroporations Hippocampal Electroporations Longitudinal Signal Detection Biological Effects Behavioral Investigations DISC1 Gene
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