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Korrelativ mikroskopi til 3D Strukturel analyse af dynamiske vekselvirkning

Article DOI: 10.3791/50386-v • 13:43 min • June 24th, 2013
June 24th, 2013 •
1, 1, 1, 2, 2, 1
1Department of Structural Biology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

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Vi beskriver en modsvarende mikroskopi metode, der kombinerer high-speed 3D live-cell lysstofrør mikroskopi og høj opløsning cryo-elektron tomografi. Vi demonstrerer evne til korrelativ metode imaging dynamisk, små HIV-1 partikler vekselvirker med værts HeLa-celler.

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Bioteknik Molekylærbiologisk Institut Strukturel Biologi Virology Biofysik cellebiologi fysiologi medicin Biomedical Engineering infektion Mikrobiologi teknologi industri landbrug Life Sciences (General) korrelativ mikroskopi CryoET Cryo-elektron tomografi Konfokal live-cell imaging Cryo-fluorescens lysmikroskopi HIV-1 capsid HeLa celle celle virus mikroskopi billedbehandling
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