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Immunology and Infection

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JoVE Journal Immunology and Infection
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
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Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

Article DOI: 10.3791/50926-v • 11:25 min • December 26th, 2013
December 26th, 2013 •
1, 1,2, 1, 1, 1
1Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes (URMITE), CNRS UMR 7278, INSERM U1095, Aix Marseille Université, 2Service de médecine interne, post-urgence et thérapeutique, Hôpital de la Timone

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This protocol describes the use of whole-cell MALDI-TOF mass spectrometry on eukaryotic cells. Here, we illustrate the accuracy of this technique by analyzing the multiple activation states of macrophages in response to their microenvironment.

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Whole-cell MALDI-TOF Mass Spectrometry Accurate Rapid Method Analyze Modes Of Macrophage Activation Chemistry Biochemistry Medicine Molecules Biomarkers Microbiology Identification Bacteria Clinical Samples Eukaryotic Cells Cell Type Identification Quality Control Assessment Diagnostic Applications Polarization Phenotypes Cytokines Heat-killed Bacteria Macrophage-specific Fingerprints Proteomic Responses Accuracy Simplicity Immune Host Response Pathological Conditions
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