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Tillämpning av retinsyra Skaffa osteocyter kulturer från Primary Mus Osteoblaster
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Tillämpning av retinsyra Skaffa osteocyter kulturer från Primary Mus Osteoblaster

Article DOI: 10.3791/51465-v • 07:13 min • May 13th, 2014
May 13th, 2014 •
1, 2, 1, 1, 1, 3, 1, 1, 1, 1
1Renal Research Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 2Department of Nephrology, Dialysis and Renal Transplant, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 3Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences, University of Trieste

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Behandling av primära mus-osteoblaster med retinsyra producerar en homogen population av förgrenade celler som bär morfologiska och molekylära egenskaper hos osteocyter. Metoden övervinner svårigheterna att erhålla och bibehålla primär osteocyter i kultur, och kan vara en fördel att studera celler som härrör från transgena modeller.

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Cellulär Biology cellbiologi cellodling ben retinsyra primära osteoblaster osteocyter celldifferentiering mus calvaria sclerostin fibroblasttillväxtfaktor 23 mikroskopi immunofärgning
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