Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Sylvia T. Cheung; Soroush Shakibakho; Eva Y. So; Alice L-F Mui

doi:10.3791/52807

Journal

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Biologia

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RAW264.7 transfecção de células com um gene repórter de luciferase

JoVE Journal
Biologia

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JoVE Journal Biologia

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

RAW264.7 transfecção de células com um gene repórter de luciferase

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20,520 Views

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12:58 min

•

June 18, 2015

DOI:

10.3791/52807-v

DOI:

10.3791/52807-v

•

12:58 min

June 18, 2015

•

18 Views

Sylvia T. Cheung^1,2,3, Soroush Shakibakho^1,2, Eva Y. So^1,2, Alice L-F Mui^1,2,3

¹Immunity and Infection Research Centre,Vancouver Costal Health Research Institute, ²Department of Surgery,University of British Columbia, ³Department of Biochemistry and Molecular Biology,University of British Columbia

Summary

Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

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RAW264.7 transfecção de células com um gene repórter de luciferase

Summary

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