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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Article DOI: 10.3791/53827-v • 11:02 min • May 27th, 2016
May 27th, 2016 •
1,2, 1,3, 4, 5,6, 7, 1,3,8,9, 1,3,8,9,10,11
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Pharmaceutical Sciences, University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, 6Magee Women's Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, 8Medicine, University of Maryland School of Medicine, 9Pathology, University of Maryland School of Medicine, 10Pharmacology, University of Maryland School of Medicine, 11Experimental Therapeutics, University of Maryland School of Medicine

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With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

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Alternative Promoter Usage Transcriptional Regulation Murine Bcrp1 Luciferase-based Reporter Assays MRNA Expression Control In-silico Methods Alternative First Exons Genetic Data Analysis Reporter Constructs Gene Regulatory Field Functional Activity Ensembl Genome Browser FASTA Format Blast Homepage NCBI Website ESTs
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