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JoVE Journal Genetics
siRNA Transfection e l'EMSA Analisi su appena isolate umani dei villi citotrofoblasti
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siRNA Transfection e l'EMSA Analisi su appena isolate umani dei villi citotrofoblasti

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

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Questo protocollo descrive un metodo per trasfezione efficiente siRNA in appena isolate citotrofoblasti villi umani con microperforazione e identificando complessi DNA-proteina in queste cellule. cellule trasfettate possono essere monitorati da Western Blot e EMSA analisi durante il tempo della cultura di 4 giorni.

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Genetica la biologia molecolare l'emissione citotrofoblasti villi umani sincitina-2 siRNA microperforazione trasfezione a base lipidica elettroforetica Mobility Shift Assay
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