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JoVE Journal Biochemistry
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli
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Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Article DOI: 10.3791/54458-v • 10:21 min • November 16th, 2016
November 16th, 2016 •
1,2, 1, 1
1Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, 2Department of Emergency Medicine, Indiana University of School of Medicine

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Resumo
Automatic Translation

A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

Tags

Protein Packaging Outer Membrane Vesicles Escherichia Coli Design Production Purification Enzyme-filled Vesicles In Vitro Catalytic Reagents Enzyme Isolation Technique Enzyme Stability Organophosphate Compound Directed Packaging Enzyme Activity Therapeutics Development Remediation Cell-free Catalytic Systems OMV Purification Microbial Cell Cultures
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