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Ploïdie Manipulatie van zebravis embryo's met Heat Shock 2 Treatment

Article DOI: 10.3791/54492-v • 11:19 min • December 16th, 2016
December 16th, 2016 •
1, *1,2, *1,3, 1
1Laboratory of Genetics, University of Wisconsin, 2Department of Neurobiology, University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program, University of Pittsburgh School of Medicine
* These authors contributed equally

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Een gewijzigde protocol voor ploïdie manipulatie maakt gebruik van een heat shock naar een één-cyclus kraam in cytokinese induceren in de vroege embryo. Dit protocol wordt gedemonstreerd in de zebravis, maar kan voor andere soorten.

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Developmental Biology zebravis ploïdie manipulatie hele genoom duplicatie gynogenese heat shock centriole duplicatie homozygote diploïde
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