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JoVE Journal Biochemistry
En optimeret protokol for elektroforetisk mobilitet Shift Assay brug af infrarød fluorescerende farvestof-mærkede oligonukleotider
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En optimeret protokol for elektroforetisk mobilitet Shift Assay brug af infrarød fluorescerende farvestof-mærkede oligonukleotider

Article DOI: 10.3791/54863-v • 09:58 min • November 29th, 2016
November 29th, 2016 •
1, 1, 1
1Department of Biological Sciences, University of Illinois at Chicago

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Vi beskriver her en optimeret protokol af fluorescerende elektroforetisk mobilitet Shift Analyser (FEMSA) ved hjælp af oprensede SOX-2 proteiner sammen med infrarøde fluorescerende farvestof-mærkede DNA-prober som en case til at tackle en vigtig biologisk spørgsmål.

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Biokemi Fluorescerende EMSA infrarød fluorescerende farvestof infrarød imaging system, HMG-box transkriptionsfaktor postmitotiske neuronale identiteter
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